High Performance Liquid Chromatography

High-performance liquid chromatography (HPLC) is one of the most versatile analytical techniques and complements gas chromatography (GC) by enabling the separation, identification, and quantification of compounds that are non-volatile, thermally unstable, or have high molecular weights. HPLC separates compounds in the liquid phase using high-pressure pumps to drive a solvent through a packed chromatographic column. This makes HPLC particularly suitable for analysing pharmaceuticals, organic acids, sugar, food additives, environmental contaminants, and metal–ligand complexes. The Vanquish HPLC equipped with UV–Vis, refractive index and charged aerosol detectors provides excellent sensitivity, selectivity, and quantitative accuracy.

A key strength of HPLC is its flexibility in method development, allowing optimisation of mobile phase composition, pH, gradient profiles, and column chemistry to separate complex mixtures with high resolution. It can analyse compounds over a broad polarity and molecular weight range with minimal sample preparation, making it indispensable in research, quality control, environmental monitoring, forensic science, and clinical diagnostics. Modern HPLC systems also support automated sample handling, high reproducibility, and robust routine operation.