Quantitative PCR

Quantitative PCR offers two analytical modules for the microbial quantification such as the abundance of environmental bacteria and fungi.

Bio-Rad CFX384

The Bio-Rad CFX384 Real-Time PCR detection system is composed of the CFX384 Optical Reaction Module and the C1000 Touch Thermal Cycler Chassis. The module is a member of the family of interchangeable reaction modules that can be used with the C1000 Touch Thermal Cycler Chassis for PCR and real-time PCR. All modules can be quickly inserted and removed from the cycler chassis without tools.

The CFX384 Touch Real-Time PCR Detection System has six independently controlled thermal electric units providing even, precisely controlled temperatures at all times during the run, including ramping.
The optical system, with five filtered LEDs and five filtered photodiodes, collects data from all wells, detecting up to four targets per well. For single-color FAM and SYBR® Green I, the fast scan option reads single-channel fluorescence in all 384 wells in just 8 seconds. A channel with an LED filter–photodiode combination is dedicated to FRET singleplex experiments.

*Bacterial abundance quantification based on the 16S rRNA gene.
*Fungal abundance quantification based on the ITS region.
*Customized service e.g. functional genes quantification.

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